A FASTQ file is a text file that stores the sequence data from clusters that pass the flow cell's filter. Demultiplexing is the first phase in creating a FASTQ file if specimens were multiplexed.

I have SmartSeq3 data that has been de-multiplexed to produce 192 pairs of fastq files with no fastq files provided for the indices. Is it possible to run 'kb count' on this data. I do not have access ...

Welcome to Day Four of my 21-day project series! Today I’ll make Your Motivator With Python And Unsplash with a GUI interface in python. Programming is not a straight easy path we all feel depressed ...

Abstract: With the continuous development of sequencing technology scientists in the cost of DNA sequencing in reduce gradually, it also makes the number of DNA sequencing data to increase ...

We introduce Single-cell Nanopore Spatial Transcriptomics (scNaST), a software suite to facilitate the analysis of spatial gene expression from second- and third-generation sequencing, allowing to ...

FASTQ format files are produced by nearly all modern sequencers and serve as a starting point for the bioinformatic analysis of next-generation sequencing (NGS) data. Understanding this format is key ...

Most scientific journals require scientists to make their sequencing data publicly available. This way, other researchers in the world can download the raw data and ...

Quality control and preprocessing of FASTQ files are essential to providing clean data for downstream analysis. Traditionally, a different tool is used for each operation, such as quality control, ...